 | | How does Qlucore calculate the length of the gene to do the normalization? | | | Question | | How does Qlucore calculate the length of the gene to do the normalization, i.e., after receiving BAM files and BEFORE TMM?
A gene, such as p53, may have many isoforms which have different exons. So how does Qlucore calculate the gene length in order to do the normalization? Taking the longest, the shortest or the union of the exons or something else?
| | Answer | Qlucore computes the gene lengths from the union of all isoforms for the gene. We use the “entire exon length” of each gene.Overlapping exons are collapsed to a disjoint union of intervals.
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